Trichothecene production in Fusarium goolgardi
- Start date:
- 01 Jan 2012
- End date:
- 31 Dec 2016
- Liliana Rocha
- Project sponsors:
- The Royal Botanic Gardens and Domain Trust
- Project partners:
- Robert Proctor and Susan McCormick (USDA, Illinois, USA)
- Assess mycotoxin production by F. goolgardi, particularly type A trichothecenes.
- Determine the phylogenetic relationships of its TRI genes.
- Analyse the coding regions of the TRI genes.
- Assess trichothecene production of F. goolgardi in planta in association with disease symptoms
Fusarium goolgardi, a recently described species associated with dieback of Xanthorrhoea glauca in natural ecosystems of Australia, is tested for its ability to produce type A trichothecenes. The phylogenetic relationships between F. goolgardi and other type A trichothecene producing species are also analysed for RPB1-RPB2, TRI core gene cluster, TRI1-TRI16 and TRI101 data sets. F. goolgardi is divided into two groups based on toxin production: one produces 3, 15- diacetoxyscirpenol (DAS), neosolaniol (NEO), acetyl- neosolaniol (Ac-NEO) and T-2 toxin (T-2) while the other produces DAS only. Phylogenetic analyses reveal that these two chemotypes are separated into two distinct clades in the TRI gene phylogenies. Research is currently underway to assess the toxin production in planta in association with dieback symptoms.